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The second validation set consisted of first lactation test day records from 35293 Jersey cows, sired by 364 Jersey bulls, a different breed of dairy cattle.
The first validation data set consisted of first lactation test day milk yield records of 23603 cows sired by a different set of 453 Holstein bulls, none of which sired cows in the discovery data set.
The discovery data set consisted of first lactation test day milk yield records of 62343 Holstein Friesian cows sired by 798 sires, milking across the range of environments of dairying in Australia, from the Australian Dairy Herd Improvement Scheme (ADHIS) database.
Approximately 13 million first lactation test day records for protein yield, fat yield, and milk yield from daughters of the sires were used.
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The daily milk yields from these simulated lactation curves were subset to equally spaced (weekly, monthly and quarterly) and unequally spaced (with four, five or six records per lactation) test-day intervals.
In 1996, Gengler [ 11] provided a review of many common definitions of persistency, which included ratios of an early test day or period to late-lactation test-day or period and measures formulated to be independent of total yield.
Random effects of animals and fixed effects of lactation stage, test day, and parity on milk yield, quality, and cortisol concentration were analyzed using mixed models.
We also compare male juvenile associations with males' previous associations with the juveniles' mothers during lactation to test the hypothesis that males may use behavioral cues from previous friendships to determine patterns of investment in juveniles.
The difference between proportion of moderate and severe signs in early (first 14 days post partum) and later lactation was tested using the Chi-square test.
Therefore, since the maximal differentiation state of the breast occurs during lactation, we tested the hypothesis that HEX nuclear localization could be modified in lactating mammary gland.
Therefore, in order to assign a biological role to increased HEX nuclear localization during lactation, we tested whether HEX was able to activate NIS promoter.
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