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Mammary biopsies were obtained from a subset of cows at approximately d 7 and d 14 of lactation, processed and stored appropriately for subsequent analyses including RNA extraction and assessment of cell proliferation and apoptosis by immunohistochemical procedures.
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Also evaluated are the integrity of the lactation process and the quality of care for her young provided by the mother.
The latter is particularly critical, since it is strongly involved in the metabolism of the mammalian organism and has a direct qualitative and quantitative influence on the lactation process [1, 11, 12].
The fat content of reindeer milk normally varies from 11 to 30% during the lactation process [ 27].
Myoepithelial cells surround the alveoli and provide mechanical stimuli to the epithelial cells of the alveoli during the lactation process.
The lactation process is shown to be delayed in mothers with diabetes (11), and their infants have a more immature sucking pattern (12).
Our results and these reports suggest that these highly abundant miRNAs may be implicated in the lactation process, milk synthesis and in mammary gland biology.
RNA extracted from cells shed into milk during the lactation process provides a good representation of gene expression in the mammary gland [ 95] and therefore eliminates the need for destructive tissue sampling.
The elements generated by this study will now be used as references to decipher the regulation of miRNA expression in the goat mammary gland and to clarify their involvement in the lactation process.
Our focus was on themes that differed across regions and not those that emerged from interviews in all regions, such as the importance of having updated knowledge about lactation processes, the training of personnel, antenatal information of the mothers, and the practice of skin-to-skin care and peer-to-peer support.
However, the induced lactation process is usually more successful in women who have previously been pregnant.
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