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The results show that, accompanied with CO2 fixation, C. cellulovorans produced lactate at a constant rate after 2 days (Figure 4b).
But a lactate level ≥4.0 mmol/l has 4.25 times increased possibility of admission per unit increase in lactate at a 95% CI of 2.77 to 6.52 (p < 0.001).
At univariate analysis, however, lactate above 2.0 mmol/l had 1.19 times risk of death within 72 h per unit increase in lactate at a 95% confidence interval of 1.14 to 1.24.
Multivariate analysis showed the possibility for non-discharge within 72 h to be 3.70 times per unit increase in lactate at a 95% confidence interval of 0.81 to 16.80.
The adjusted model that also includes a lactate level ≥2.0 mmol/l has 1.75 times increased possibility of admission per unit increase in lactate at a 95% CI of 1.11 to 2.75, but the level of significance is less (p = 0.02).
In the flow-through experimental run, NAu-1 (200 mg dry weight) and S. oneidensis cells were loaded into a 25-mm-diameter plug-flow reactor supported by a 0.2 μm membrane filter (polyethersulfone membrane), and subsequently exposed to a flow of O2-free 25% Ml media (including 5 mM lactate) at a flow rate of 0.3 ml min-1.
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Both strains also utilized maltodextrin derived from cassava starch and produced d-lactate at a concentration of 33 34 g/L with yields of 0.91 0.92 g/g maltodextrin utilized.
The frequency of measuring lactate (at any time and within 6 h) and the time to obtaining lactate all improved.
Of note, lactate values at inclusion were not associated with lactate evolution, strengthening the concept that an elevated lactate value at a given time point does not infer the presence of anaerobic metabolism.
HBE analysis resulted in high classification accuracy with 6 genes which are DNA replication licensing factor CDC47 homolog (D55716-at), gamma-interferon-inducible protein IP-30 precursor (J03909-at), LDHA lactate dehydrogenase A (X02152-at), CD69 antigen (Z30426-at), SLC (AB002409-at), Rad2 (HG4074-HT4344-at).
A lactate solution at a concentration of 50~500 mg L−1 made from dissolving lactate sodium salt in deionised water (DI) water was used as standard.
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