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Free logic lacks this restriction and so countenances empty names as in 'Sherlock Holmes is a detective' and negative existentials such as 'Pegasus does not exist'.
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Due to single nucleotide substitution, the corresponding region of rZH501-M847-G lacked this restriction enzyme site.
Also, this approach should translate well to even the largest eukaryotic genomes, however some gaps in coverage would be expected in long stretches of low complexity sequence that lacks the restriction site.
The corresponding sequence of B. divergens lacks the restriction site.
The polymorphic allele CYP2D6*4 lacks the restriction site and is thereby retained as one fragment.
The wild-type G allele was digested into 461 bp and 27 bp fragments, while the C allele, which lacks the restriction site, remained uncut.
C t) values were normalised to a non-relevant amplicon lacking the restriction site.
Haplotype analysis revealed that the most prevalent haplotype lacked the restriction sites for all five RFLP markers.
Results from Figure 9 demonstrate that IFN plays an important role in limiting RSV infection in RWPE-1 and PC-3 cells, whereas lack of this restriction in LNCaP cells associated with excessive viral replication and oncolysis.
As from agarose gels, it was observed that both polymorphisms exhibited the corresponding single-band pattern of each allele: the LEP allele A that lacks HhaI restriction site was defined by a 451 bp fragment, while allele G, which contains this restriction site, was characterized by two digested bands of 402 and 49 bp.
A partial ALS gene fragment was amplified with forward primer 5′-GCTGCTGAAGGCTACGCT-3′ and reverse primer 5′-CACTTCTTGACTCAGTCCCGC-3′ and digested with EcoRV to confirm the presence of ALS alleles from A. tuberculatus (contains an EcoRV restriction site) and A. palmeri (lacks restriction site) (Tranel et al. 2002).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com