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Finally, of 173 MIRNA loci, 17 completely lacked sequence coverage in at least one strain.
Altogether 70 primer pairs within 8kb of GAD1 proximal promoter and 5'end sequences were tested; 67 primers pairs produced amplicons that lacked sequence specificity (data not shown).
This analysis in addition to sequence conservation showed that putative HRE's 1 4 were potential candidates for hypoxia regulatory elements, while HRE-5 lacked sequence conservation and was not further examined by experiments.
Moreover, many co-expressed genes with the highest A/ S ratios lacked sequence conservation with known genes, suggesting that they may be specific or highly diverged in spruce.
Secondly, every positive viral amplicon identified was confirmed by Sanger sequencing; any amplification that lacked sequence verification was considered a false positive and excluded from the analysis.
This is because until recently there were limited data on linear organelle DNAs, and those that were available often lacked sequence information from the telomeres and subtelomeres.
Similar(50)
Phylogenetic studies investigated this phenomenon but have lacked sequences from more subtropical and tropical regions, particularly from Southeast Asia.
This 50% is actually a conservative estimation because we excluded the transcripts that lacked sequencing depth.
Most of these minor OTUs lacked sequences from American individuals (e.g. OTU006, 007, 011, 013, and 014).
We also removed the parts of the alignments that lacked sequences from any of the three species.
They often lack sequence conservation and have undergone rapid evolution in higher organisms.
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CEO of Professional Science Editing for Scientists @ prosciediting.com