CtpAp cleavage of both substrates yielded multiple peptide fragments of different sizes, suggesting that cleavage by CtpAp lacked stringency in site selection on each substrate.
This again raises the question of the possible lack of stringency of pluripotency tests in human, and also highlights the differences observed from 2 lines obtained in similar conditions, and the fact that each line, to a certain extent, probably follows its own path of reprogramming.
The occurrence of cDNA synthesis in absence of RT primers or in the presence of false primers was documented previously [ 2], and it was attributed to a lack of stringency causing false priming or priming by other RNA molecules present in solution along with the target RNA [ 2].
In consequence, many studies, fueled by commercial self-interest, have lacked the stringency necessary for true scientific advancement.
The -p option increased the sequence identity necessary with overlaps to 90 from a default of 75, and thus was found to be lacking in stringency.
Furthermore, the lack of generality and stringency in definition of terms such as "main force-generating step" and "power stroke" contribute to the problems.
For forensic as well as for phylogenetic purposes we have already successfully established evaluated sequencing strategies that proved to be useful in a number of investigations where precise base-calling was necessary for the CR [ 22- 26], however such stringency is lacking for the whole mt genome.
Without an ability to understand the potential benefit (or lack thereof) of the additional stringency, an appropriate consideration of benefits and costs is not possible.
Colonies sequentially co-transformed with pGBKT7-CCR2B47 and various constructs encoding FLNa repeats were plated out onto low stringency SD plates (lacking Trp and Leu).
Transformant-colonies grown on high stringency selection media lacking, tryptophan, leucine and histidine (SD-T-L-H) were subjected to a colony-lift filter assay.
