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Lack of tick control measures on animals grazed in infested areas and the poor animal husbandry practices may be other factors for the observed high tick loads.
Generally, the tick challenge recorded during this study could be attributed to the more conducive conditions for tick proliferation, survival and lack of tick control programs in most pastoralist communities in Tanzania [ 39].
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The lack of ticks in ground traps compared to canopy traps could suggest differences in host acquisition strategies between the two environments.
The lack of known tick expressed gene coding regions and the abundance of retroviral sequences from these two randomly selected BACs suggests a rather low tick expressed gene density in the R. microplus genome.
Mechanical transmission in the absence of ticks and lack of transmission through ticks are questions that need to be addressed.
Lack of specificity of tick DNA template kit was confirmed experimentally.
In 2010, in collaboration with Raoult (4 ), we posed a question in Emerging Infectious Diseases, "Could ticks transmit Bartonella spp.?" That article elicited an editorial response emphasizing the lack of evidence supporting tick transmission of Bartonella spp. (5 ).
Although clinical findings of our patients were comparable with those of the Missouri STARI patients, our patients' multiple, widespread, and oblong lesions with no punctum and lack of known histories of tick bite were comparable with those of New York LD patients (3 ).
It shows the lack of statistical differences between tick intensity in high-impact anthropogenic areas (HIAA), low-impact anthropogenic areas (LIAA) and mixed areas designed, in an objective way, by GIS techniques.
The reason could be the lack of data from unfed tick salivary glands as only cDNA libraries from unfed Argas monolakensis and Amblyomma americanum were published to date in the GenBank.
A role in transmission is not immediately apparent for these genes, in fact, it is not surprising that more than half of the candidates were of unknown function due to the lack of information on the determinants of tick transmission.
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