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Although at the summary statistic level we did not replicate the lack of localisation information, we did find trials on which detection responses were correct but those for localisation were incorrect.
A null localisation effect could, for example, be due to less precision in the localisation task than the detection task due to the additional requirements rather than a true lack of localisation information.
These findings suggest that the apparent lack of localisation on some trials where a mass was detected is, at least in part, driven by image variability, such as small masses in a proportionally large breast and normal tissue with salient features (distractors), and response imprecision.
Thus, the lack of localisation of transcripts of any of these 19 genes during oogenesis or early embryogenesis suggests that Oncopeltus lacks germ plasm.
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While there seems to be conflicting evidence in this regard, the observed lack of nuclear localisation could reflect either the cell type used, or the reduced nuclear localisation activity of human PLCζ compared to mouse.
Therefore, we can conclude that the lack of nuclear localisation of PB2 mutants is not the main cause for the lack of RNP activity.
Here there was lack of significant localisation of MTZ to the nucleus.
Lack of BubR1 localisation to kinetochores has been associated with the mitotic spindle checkpoint inactivation and increased cell death (Dai et al, 2004; Lee et al, 2004).
This proposal is reinforced by the lack of co-localisation of Cx45-eGFP cells with GAD-67, a GABA-synthesizing enzyme that is expressed in GABAergic interneurones (Heinke et al. 2004).
The presence of some punctate labelling in a part of the cell between the kinetoplast and the flagellar pocket zone might suggest an association with early endosomal compartments, but the lack of co-localisation with endocytosed dye FM4-64 (data not shown) argue against this.
The key limitation is the lack of control on localisation, density and size uniformity of the structures.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com