Some of the most important changes after EtOH exposure are the mitochondrial morphology alterations, the lack of crests and the presence of heterogeneous bodies surrounded by ER.
Hachisuka believed the carnivorous habits described by Tafforet to be unlikely for a starling, and thought the lack of a crest suggested that it was not closely related with Fregilupus.
A report by Yoshida, clearly shows the lack of neural crest cell marker, X-gal staining in the parietal bones of Wnt1-cre/R26R mice [63].
Despite the apparent lack of neural crest defects in the trunk of WAF-treated embryos, there was reduced circulation in the intersegmental blood vessels, and those vessels devoid of any circulation often showed excessive vascular branching.
Although two lineage analysis studies in mice suggested that a small subpopulation of unspecified olfactory sensory neurons may have a neural crest origin (Forni et al., 2011; Katoh et al., 2011), they relied upon Wnt1-Cre and P0-Cre, both of which suffer from lack of neural crest specificity.
Currently, mammalian experiments are severely limited by the lack of neural crest-specific labeling and the difficulty of live cell tracking.
'Moderate' embryos have near complete lack of neural crest-derived microvillous neurons, whereas ciliated neurons are present and project to the olfactory bulb.
Previous research has shown that homozygous crested chickens are distinguishable from heterozygous chickens by the lack of filoplumes in the crest.
This favourable scenario might have been created by the lack of maintenance of the crest drainage channel, adjacent to the slide area.
The authors interpreted their negative results to indicate a lack of contribution of neural crest cells to the olfactory epithelium.
