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The deleterious effects of these human COQ6 mutations were validated by their lack of complementation in coq6-deficient yeast.
Another concern is a lack of complementation.
There are several explanations for the lack of complementation.
To discard that lack of complementation stems from poor chimeric-protein stability, we tested the steady state levels of these proteins by Western blot, using antibodies raised against the C-terminus of Swf1, which is shared by all the constructs.
Lack of complementation on SLAD medium may indicate haploinsufficiency of HOS2, as reported previously for other mutants grown on SLAD, such as gap1Δ/Δ and gpr1Δ/Δ [28], [28].
Retrograde passage is halted at one synapse due to lack of complementation in premotor neurons.
Similar(29)
The lack of full complementation is most likely due to insufficient induction due to instability of the inducer.
Alternatively, lack of full complementation by yheF could be attributed to expression of yheF from an artificial promoter on the plasmid.
It should be noted that the Sec-dependent substrate ssPhoA-Y1, like its ssDmsA-Y1 counterpart, accumulated in both the cytoplasm and the periplasm of wt cells (Fig. 2c), indicating that the lack of YFP complementation for the ssPhoA-Y1 construct was not due to poor expression/stability or to highly efficient translocation via the Sec pathway.
The lack of full complementation by the ssrA-DD allele cannot be explained by reduced expression of the mutant tmRNA relative to wild type because Northern blot analysis suggests that it is expressed at the expected level when present on a single- or multi-copy plasmid (see Figure 1B).
Thus, lack of genetic complementation for spike branching in the F1 progenies further indicates that the same L96P mutation at bh t may be the casual factor for the branch phenotype.
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