Another is the lack of amplification, although a mike might have helped Ramon Vargas, a careful, smallish Rodolfo, to be heard.
Another is the lack of amplification, which shouldn't be a problem for the fine soprano Hei-Kyung Hong as Mimi and the veteran pro Frank Lopardo as Rodolfo.
If anything, the lack of amplification heightened the sense of intimacy that has been one of Le Poisson Rouge's attractions.
The lack of amplification was a reminder, if any were needed, of rural Vermont, where they met at Bennington College and began singing together at house parties just two years ago.
Preliminary results suggest a lack of amplification competent prions in samples processed by alkaline digestion at 2, 4, and 6 h cycles as compared to nondigested samples.
Within five days, approximately 50% of tests showed a total lack of amplification of the calibrator and in most cases also of the HIV-1 template.
Protein targets in particular are especially challenging to detect quantitatively and sensitively due to the lack of amplification strategies akin to nucleic acid amplification.
The lack of amplification could indicate either a lack of nitrogen fixation or poor primer specificity for this community, though multiple qPCR assays were attempted to optimize for amplification if the genes were present.
The abundance of these fungal microorganisms in the tissue culture material is also likely to be very low based on the low number of filaments observed microscopically and the lack of amplification of fungal DNA using single primer sets.
Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa.
In an earlier study, it was found that amplification of both bands corresponding to the insert and AOX1 gene was used as indication of Mut+ phenotype, while Muts was distinguished by the lack of amplification pertaining to native AOX1 gene (Linder et al. 1996).
