These observations support our hypothesis that cIntFams represent broadly-conserved nematode characteristics, while also inferring that about two thirds of the cIntFams (1990) conserved within nematodes lack conservation across all of the host and outgroup species.
The expansion and evolution of microRNAs have been linked to both body plan innovation and vertebrate morphological complexity throughout evolution [21], [22], [23], suggesting that some newly-described microRNAs may lack conservation with distant species.
Comparative analysis with representative homologues belonging to all realms reinforce the fact that ferritins are dynamic protein cages and lack conservation vis-à-vis residues defining gated channels/pores that control flow of iron, oxidants and reductants.
The regions with highly repeated content lack conservation in the gene order and their gene density is very low.
Neither of these SNPs is located in the HD1 or ATP/Mg2+ patches identified and lack conservation across different species.
Notably, cellular internal ribosome entry sites lack conservation of both the primary sequence and mRNA secondary structure, rendering their identification difficult.
Although angiosperm AARs lack conservation and 3D structure, a fraction of the proteins that contain AARs may be functionally important and are under extensive regulation in plant cells.
In addition, one has to keep in mind that spurious association of ribosomes could lead to translational noise and as such most of the transcripts suggested to encode small peptides seem to lack conservation of their proposed coding regions [ 25].
