Sentence examples for lace p from inspiring English sources

Alanine fed animals globally expressing dSpt1 C129werere used to confirm the identity of the DSB peaks and homozygous dSpt2/ lace P hypomorphs were used as positive controls to identify endogenous sphingoid bases in the wild-type traces based on their absence in dSpt2/ lace P samples (previously shown to have a sphingolipid content of 2.58% (25)).

Introduction of heterozygous alleles of dSpt1 or dSpt2/ lace P in the presence of the neuronally expressed dSpt1 C129W induced a reduction of bouton number of 50%, phenocopying the homozygous loss-of-function allele of dSpt2/ lace P (Fig.  1B and C).

This genetic interaction between dSpt1 C129W expression and heterozygous mutations of dSpt1 and dSpt2/ lace P strongly suggests a dominant-negative function for the dSpt1 C129W mutation when examined in the context of lethality and synaptic growth.

The lace P mutant is a hypomorphic transposon insertion in the dSpt2 locus that as a homozygote dies early in the pupal stage of development (21) and has been shown to express 2.58% of wild-type sphingolipid levels (25).

Stocks used in this study: UAS-d Spt1 C129W, UAS- Spt1 (this study), UAS- ChRh2 (56) (Gero Miesenböck, Oxford), UAS- LYZ-GFP-KDEL (33) (Mary Lilly, NIH, Bethesda), UAS-Tetanus-toxin (29), pain (28) (Dan Tracey, Durham, NC), lace P (21) (John Roote, Cambridge), SPT1 l(2 SH1626 (19) (Stephen Hou, NIH, Bethesda), pickpocket-GAL4 (26) (Wes Grueber, Columbia).

We also find that we can rescue synaptic structure in an Elav-GAL4/UAS- dSpt C129W, lace P animal by feeding serine in the diet suggesting that the potential dominant-negative action of dSptC129W may be due to incorporation of alanine in nascent sphingolipids.

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These results were significantly superior to other cisplatin-based combination chemotherapy regimens used in the LACE meta-analysis (P = 0.04).

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Interestingly, feeding of serine can rescue the synaptic development phenotype in an Elav-GAL4, UAS- dSpt1-C129W, lace P/+ animal (Fig.  3E and F).

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