Adherent methods currently employed for ES cell culture are unable to provide a suitable culture system due to the heterogeneous static conditions, resulting in batch-to-batch variation, labour intensive methodology and ultimately, restricted cell number due to the available surface area, leading to limitations in scalability [7] [9].
Although more labour intensive, such methodology allows a more accurate assessment of lymphatic density.
However, the methodology is quite labour intensive, and design and methods are limited by stringent budgets, imposing deviations in the core protocol [ 63].
Nonetheless, researchers should anticipate that maintaining blinding by such methodology will be labour intensive, and that guarding against inappropriate unblinding must be a high priority.
However, this methodology is time consuming, labour intensive, has limited resolution and is not effective for the analysis of a large number of clones.
The most time-consuming, labour intensive and costly stage in the CRISPR-Cas9 methodology involves screening and identification of mutant cell clones.
The methodology proposed to score multidose markers is effective; however, it is labour intensive and is currently not automated, limiting its applicability.
The primary aim of this investigation was to examine target labelling methodologies that could be used for our custom miRNA microarray that did not require labour intensive steps such as gel purification, were sensitive and specific, useable at high-throughput and more affordable than multiplex RT-PCR assay.
The design and implementation of a PLM solution in a cross-company environment is a complex and labour intensive operation, which is often coupled with a Business Process Re-engineering (BPR) project to better deploy technologies as well as methodologies and to target the system implementation on the real company needs.
Dealing with Ebola is labour intensive.
