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Since more than a decade, glucose control (GC) has been an important, yet laborious treatment goal in intensive care.
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Solid sampling techniques such as glow discharge (GD) optical emission spectrometry (OES) and mass spectrometry (MS) are very attractive for industry and research since they allow fast and sensitive multielement analysis and do not require laborious sample treatment.
Neither laborious pre-treatment nor clean up were necessary.
Each of the approaches, though, possesses limitations including: power/reagent consumption and lack of portability for instrumental techniques; laborious sample-treatment steps for wet methods; and lack of selectivity and sensitivity with sensors when employed with complex samples.
In addition to the good reproducibility, operational and storage stability, the developed biosensor was successfully used for the determination of phosmet in olive oil samples without any laborious pre-treatment.
Users take advantage of the direct test format by avoiding laborious sample pre-treatment as well as addition of costly reagents, both being common disadvantages of competing test systems.
Due to these limitations, in general only low filler loadings can be achieved without compromising the separation performance unless laborious filler post-treatments are applied.
The general method for supernatant collection was time consuming and laborious for the sample treatment, especially for the samples from 48-deep MTPs which was impractical for rapid and high-throughput screening.
Determination of pure β- and α-emitters is much more laborious, as it requires chemical treatment and separation.
Studies with laborious protocols or where the treatment arms are very different, e.g. chemotherapy or not, or where there are restrictive patient trial entry criteria tend to have more problems with patient recruitment; as illustrated by the 73% refusal rate of eligible patients in a multi-modality NSCLC trial (Spiro et al, 2000).
Nevertheless, the current DNA methylation detection approaches require bisulfite treatments and are laborious or costly to perform.
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