That's 280 hearings and, assuming conservatively that each hearing lasts four hours, over 1,100 laborious hours targeted at the Obama administration and the Democrats, whether or not the subjects are worthy of investigation, let alone of any value to the American public.
Although newer, and arguably less laborious, strategies to target genes [24] or to tag proteins utilizing novel recombineering strategies [25] are now becoming available, it is important to note that our reporter does represents the first faithful reporter of ADGF-A expression to be published.
This is due to limitations in the sample preparation caused by failure to focus or laborious protocols used to target the sequencing process to the aim of the work.
Customized techniques such as massively parallel DNA sequencing undoubtedly have the advantage of high sensitivity (2) but are laborious and costly, currently target only NLRP3, and are not widely available in nonspecialized laboratories.
However, in the model organism Caenorhabditis elegans non-transposon mediated gene targeting remains laborious, and as a result has not been widely used.
Since experiments used for the identification of miRNA targets are laborious and are hard to apply for large-scale investigations, many computational approaches have been recently developed to predict miRNA targets in vertebrates and Drosophila[31] [38].
As experimental techniques of the time were both laborious and expensive (see "The targets you see depend on the targets you look for"), quite reasonably, experimental validation has focused around those interactions most likely to yield positive results, leading to a positive reinforcement in the literature dominance of the seed site.
In addition to the advantages of scalable, affordable, and easy to engineer, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) technology is superior for multiplex targeting, which is laborious and inconvenient when achieved by cloning multiple gRNA expressing cassettes.
Often the detection of the targeted event required laborious fly screening by PCR (Gratz et al. 2013b; Yu et al. 2014; Gokcezade et al. 2014).
The double-joint polymerase chain reaction (DJ-PCR) is a technique that can be used to construct vectors for targeted genome integration without laborious subcloning steps.
