Sentence examples for laborious protocols from inspiring English sources
It can also assist more laborious protocols, e.g. paired end libraries, with end-polish, ligation, immobilization, fill-in-reactions etc., alleviating work load and making it possible to prepare more samples in parallel.
This is due to limitations in the sample preparation caused by failure to focus or laborious protocols used to target the sequencing process to the aim of the work.
The laborious protocols have to be performed by highly skilled technicians to achieve acceptable reproducibility.
In order to maintain the pluripotency of hESCs in cultures, manual microdissection is broadly used for cell passaging, which requires laborious protocols and quality-controlled manual selection [ 9].
Although these features make it highly desirable, RNA-Seq is not practical for all experiments, due primarily to laborious protocols and the need for large quantities of starting material.
Despite various improvements like LongSAGE [ 5] and SuperSAGE [ 6], issues such as lower data acquisition rates, laborious protocols, and the requirement for specialized equipment have restricted rapid adoption of sequencing-based methods.
Despite its potential interest, after this initial report, SPEX has not been widely applied mainly because of its intrinsic laborious protocol compared to other more innovative procedures (see "Next Generation Sequencing methodology" section).
It is probably not worthwhile to invest in laborious sequencing protocols such as cloning [43] to obtain barcodes for a region that can only reduce the fail rate of the BLAST test by 7 15%, and in contrast, full-length rbcL as a single DNA barcode locus seems the more practical choice.
The methods also typically rely on multiple steps including chromatography, gel electrophoresis, laborious physical extraction protocols coupled with chemical detection of liberated inorganic orthophosphate, or the use of radiolabeled polyP.
While cloning and sequencing is time consuming and laborious, our RSCA protocol can handle a high number of fish in shorter time.
In contrast, in this study we report a far less laborious and costly protocol in which whole-genome sequencing data from the complete genomes of two siblings were rapidly analyzed and compared with each other and with the reference using RelocaTE.
