Genetic modification of ES cell-derived DCs, and laborious modification of the β2m or TAP gene, has been attempted to solve the problem of HLA class I allele mismatch between human ES cells and the recipients to be treated.
While demonstrating an important advance, this approach requires laborious modification of native GLP-1 and the reaction schemes used in these studies to immobilized GLP-1 on polymer chains was a random conjugation between carboxylates of the polymer chains and primary amines of GLP-1, including its bioactive site at the N-terminus.
Eliminating restriction endonuclease activities in a number of host organisms, including Bacillus subtilis[ 23], Thermosynechococcus elongates[ 24], Borrelia burgdorferi[ 25], and Clostridium acetobutylicum[ 26], improved transformation efficiency and simplified the transformation protocols by removing the time-consuming laborious DNA modification steps.
Better carriers are now being sought to reduce the laborious and time-consuming modification of carrier surfaces, to eliminate errors that arise during weighing of the silica gel, and to eliminate adsorption of contaminants by the surface [ 11– 13].
Until recently, metabolic engineering in plants relied on the laborious testing of ad hoc modifications to achieve desirable changes in the metabolic profile.
In comparison, standard ChIP-seq protocols followed by the Illumina TruSeq ChIP-seq library protocol takes 4 5 days even with recent modifications to remove several laborious spin-column steps (Additional file 1: Figure S1).
Also, the handling and modification of retroviral vectors is laborious and time-consuming.
Modification of the Schizosaccharomyces pombe genome is often laborious, time consuming due to the lower efficiency of homologous recombination.
At present in vitro translation systems suffer from certain shortages like laborious extract preparation, low protein synthesis yields or the inability to support posttranslational modifications [ 14].
In contrast, eukaryotic systems are less productive and extract preparation is more laborious, but complex proteins can be produced more efficiently and extended post-translational modifications are supported.
In our experience, site-specific modification of patient names lists and regular expressions can be laborious and can lead to "overscrubbing" information that is valuable for research.
