Using the laborious root length method, Ma et al. (2005) and Huang et al. (2009b) screened less than 2000 rice lines, which was far from saturation, implying that more Al-sensitive or Al-resitant mutants await to be screened.
Basically, the laborious techniques we learned at length can now be duplicated with non-techniques that anyone can learn in thirty seconds.
Current library construction methods that enrich for full-length mRNA are laborious, and involve several enzymatic steps performed on mRNA, which renders them sensitive to RNA degradation.
"What really surprised me," Mr. Spronk recalled, "was the vigor and the incredible energy, how laborious these reworkings were and to what length Mondrian went to attain these paintings.
To collect these data, the usual procedure consists in laborious manual measurements on predefined traits such as panicle length, the number of branches, the order of branches, the number of grains, and grain size.
Additionally, it is a more rapid and specific method of MTC speciation apart from being less laborious compared with biochemical, phenotypic and IS 6110-restriction fragment length polymorphism (RFLP) analysis [ 22].
However, as the number of structures is exponentially related to sequence length [ 11], this method produces a large number of structures, which are laborious to analyse.
These methods can be used to improve the full-length cDNA clone content of the cDNA library, but they are all very laborious and involve several enzymatic steps that must be performed on mRNA.
Jettisoning traditional laborious in silico searching methods to define putative epitopes, we simply immunized available BChE knock-out mice with full-length recombinant BChE protein (having been produced for crystallographic analysis).
