This improves the usability of the protein since reducing agents and subsequent laborious buffer exchanges can be avoided.
In an even less partisan but more laborious crusade, he sought fixed exchange rates and a "dollar standard".
Identification of a suitable counterselection marker is complex and often organism specific (11); however, it is often assumed that isolating mutants using two-step allele exchange is extremely laborious without a counterselection marker due to the low frequency at which the second recombination event occurs (11).
The conventional procedures for soil Cation Exchange Capacity (CEC) measurement are time consuming and laborious.
The few reports to date of mutagenesis of S. suis used allele exchange without a counterselection marker and necessitated the laborious screening of large numbers of colonies (24– 24).
Before the Clearing House, banks had settled their accounts in a laborious manner, employing porters to travel from bank to bank to exchange checks for gold specie.
However, laborious handling of these adherent cells, limited scalability, and daily medium exchange prevents this production system from being suitable for production of more than a few antibodies in parallel.
The water solubility of cubic IOMNPs synthesized by means of laborious non-hydrolytic methods can be achieved through an additional step based on ligand exchange reaction as it was previously reported [18, 22, 25, 26].
There are regulatory uncertainties, the major one being whether or not Bitcoin exchanges need to get a money-transmitting license for every state, a laborious and expensive process.
Christmas at Sandringham does sound laborious, with the Master of the Household handing out schedules and room plans, and gifts being exchanged on Christmas Eve.
The currently used method for purification of His6-tagged MMP12 is a laborious procedure that requires solubilization of MMP12 inclusion bodies, refolding over multiple days, followed by anion and cation exchange chromatography [17].
