Transformation frequencies with NHEJ-deficient strains are often low, making it difficult and laborious to generate enough mutants for library screening.
While descriptive approaches can be viewed as either too laborious or not goal oriented enough, the emergence of novel testable concepts using quantitative strategies strongly suggests otherwise.
They repeatedly asked whether that was enough time to conduct laborious hand counts in the populous counties where they are now under way.
Well, I think the double-edged sword is that you sometimes feel like it's a touch laborious, but then if no one cared enough to talk to you, you would probably be a little bummed.
We still had a few hours of paddling to do the next morning, including several sets of rapids, but we were ready to crown this trip as our best yet: great weather, unspoiled wilderness, no laborious portages, no other people and countless rapids that were challenging enough to thrill but — for the first time ever — not enough to dump us.
While laser capture microdissection (LCM) can provide a relatively homogeneous sample by concentrating on the cell type of interest, generating enough sample for a conventional proteomic study is laborious with a minimum of 100,000 cells and a dissection time in tens of hours required for one 2D-PAGE [ 22].
However, at the moment such random mutagenesis experiments are laborious and functional assays for most genes are probably not accurate enough to capture the overwhelming majority of very small effects that are expected in DMEs based on population genetic inferences [ 2, 93].
Handheld blood glucometers, originally intended for use by type I diabetic outpatients in the 1980 s, are not accurate enough in the ICU environment [ 17], and are very laborious to use.
Although in Farrow's demo the Wi-Fi was turned on — common enough for iPhone users, since AT&T's EDGE network makes Web surfing slow and laborious — Moore says his exploit can work on EDGE, too.
