However, the experimental approach is generally laborious, cost-prohibitive, requires advanced molecular isolation strategies [25], and sometimes not even possible [26].
However, synthesis of siRNA having sufficient knockdown efficiency is laborious and cost intensive.
While numerous recent methods have emerged that are capable of providing base-resolution of 5-hmC, these methods are sequencing-based, making them laborious and cost prohibitive [ 10, 11].
While validation of every variant is a laborious task costing time, material and expense, we recommend that validation of actionable mutations that will directly affect patient management be performed in this kind of testing.
To circumvent the laborious and cost-intensive duplication of all processes during clone generation and quality control, we developed a cloning strategy that allows for the simultaneous generation of two variants of Gateway entry clones, one containing a particular ORF with and the other without the stop codon.
So from 2004 through 2006 he laid off 7,700 of his 35,000 employees and shut 7 of 35 plants in Europe, laborious moves that cost Bombardier $633 million.
Classical method have some disadvantages like more time consumption, laborious process and high cost, in addition to this, it fails to determine the combined effect of different factors.
Although the necessary equations for heat transfer and the pressure drop in a double pipe heat exchanger are available, using these equations the optimization of the system cost is laborious.
The use of SAGE had been until recently limited by the sequencing cost and laborious steps inherent in the cloning procedure.
Although this is time-consuming, laborious and increases product cost a little, the advantages of this measure outweighs its disadvantages as a whole.
