The laborious constructions of minigenes and the low pSPL3 efficiency precluded a more widespread use and the cloning of large minigenes with several exons [Burn et al., 1995].
Indeed, when compared to quantitative trait loci (QTL) analyses, these studies present the advantage of i) comparing concomitantly several alleles, ii) avoiding laborious population constructions and iii) exploiting the recombination events that have occurred over several generations [ 1- 3].
Onshore removal of oil that has penetrated sandy beaches and coated rocky shores is a laborious affair, frequently involving small armies of workers wielding hand tools or operating heavy construction-type equipment to scrape up contaminated debris and haul it away.
The genome of the type 1 strain M129 (ATCC 29342) was sequenced by using a laborious approach involving the construction of an ordered cosmid library.
It also speaks of labour: from the production of the sacks in south-east Asia, to their later use in the unregulated cocoa industry in Ghana, then their laborious repair in Mahama's construction of these great curtains.
Although advances in transformation efficiency and promoter usage have improved genetic studies, some basic steps in vector construction are still laborious and time-consuming.
Unfortunately, the design and construction can be laborious and uncertain.
