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Male BALB/C mice, 6 weeks old (Jackson Laboratory) were held in a room with a 12 h light 12 h dark cycle and were used at 8 weeks of age.
Male BALB/C mice and C57Bl/6 mice, ordered at 6 weeks of age from The Jackson Laboratory, were held in a room of a specific pathogen-free animal facility with a 12 h light/dark cycle, and were used at 8 weeks of age.
In O'Shea v Sullivan, a GP and a pathology laboratory were held liable for failure to detect cervical cancer in a patient who complained of intermenstrual bleeding and post-coital bleeding [ 27].
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Seawater in the laboratory was held at ambient ocean temperature (~29°C).
The illuminance level in the windowless laboratory was held constant at about 100 lux for all participants.
On their arrival in the laboratory, mice were held in standard laboratory cages in groups of 5 7 and randomly assigned to nine experimental groups (Fig. 1A and 5A).
In the laboratory they were held at temperatures between 15°C and 20°C in their original sampling jars at low density (crowding can lead to cuticular damage and water loss [31]).
In the laboratory, females were held in filtered (0.5 μm), UV-irradiated seawater (salinity of 35, hereafter referred to as culture water) at 20°C under an artificial 12 12 h light:dark (06h00 to 18h00) photoperiod until larvae hatched, which always occurred at night.
In our study, women with and without a diagnosis of fibromyalgia attended two laboratory sessions, which were held on separate days.
Live specimens of all the study species were collected directly from nests in the field then placed immediately on liquid nitrogen for transport back to the laboratory, where they were held in a −80°C freezer pending genetic analysis.
In this study we used 6-week-old male C57Bl/6 mice (The Jackson Laboratory, Bar Harbor, ME), which were held in quarantine for 12 days before the start of exposure, in an onsite, Association for Assessment and Accreditation of Laboratory Animal Care accredited vivarium in poly-propylene, fiber-covered cages in HEPA-filtered Thoren caging units (Thoren Caging Systems, Inc., Hazleton, PA).
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