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Regarding the inlet, a measured pressure profile in the catheterization laboratory was applied as a boundary condition.
In this study, cholesterol-anchored, reduction-sensitive PEG (first synthesized by our laboratory) was applied to develop a co-modified liposome with improved tumor targeting.
A quantitative sandwich hybridization assay which was developed earlier in our laboratory was applied [ 21, 22]. Figure 6 shows the levels of mRNAs encoding the procollagen II chain, AOX1, EF3 (translational factor), and C-P4H.
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To improve accessibilities of soluble PTP1B or other compounds to ETK-FL in native lipid environment and also to simply the experimental procedure, poly-styrene-maleic-acid (SMA3000 3:1) compounds (purchased from Malvern Cosmeceutics company, UK and hydrated in authors' laboratory) were applied to wrap up the lipid bilayers to form uni-lamellar lipodisq (Fig. 2A).
Radar-absorbent material (RAM), developed in a Lockheed laboratory, were applied to the aircraft's flat surfaces – for the windscreen, special coatings were applied to give them metallic characteristics.
Serial dilutions of monoclonal antibody purified in this laboratory were applied to the anti-IgG coated wells and the concentration of antibody was extrapolated from the standard curve.
This set of target congeners included more low chlorinated (more volatile) congeners than are measured in the set of 35 NHANES congeners, because the methods used by our laboratory are applied to analyze air samples, as well as biological samples.
RT-PCR and immunohistochemistry, using specific anti-HERG antibodies developed in our laboratory, were applied to tissue specimens obtained from 18 endometrial cancers and 11 non-cancerous endometrial tissues.
ABC reagent (Vectastain Elite, Vector Laboratories) was applied for 1 hour at a concentration of 9 µl/ml per reagent.
Thereafter, ABC solution (Vector laboratories) was applied again for 15 min. Followed by AEC for 2 min and Hemalaun staining.
The membranes were rinsed with Tris-buffered saline, treated with horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch Laboratories) was applied for 1 h at 1 2000 dilution.
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