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In our experience, the questionnaire, including explanations, was usually completed in less than 3 mins on initial use in the laboratory (slightly longer in clinical use), with subsequent administrations taking only 1 2 min for most subjects and patients.
Although initial length was found to be unimportant in the lake common garden analysis, we included it in this analysis because YOY in the mesocosm common garden were housed in the laboratory slightly longer; therefore, variance in initial size was greater and could have had a more substantial effect on growth.
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Each laboratory used slightly different methods, which included different extraction, detection and analytical methods.
The total number of swabs received in the laboratory decreased slightly from 60,245 in 2000 to 55,223 in 2004.
Different laboratories used slightly different names for the same SSR and RFLP markers.
The agreement between any two laboratories is slightly higher with the values around 80% (from 0.78 to 0.84).
Different laboratories used slightly different names for the same SSR or RFLP markers and assigned different letters to the multiple loci of multi-locus markers.
Therefore, many variations have been introduced into the original protocol in recent years, and most laboratories apply slightly modified protocols, which have been established based on practical experience.
First, sample preparations and spectral acquisitions were performed at two laboratories using slightly different protocols and instrumentation, with one of the labs focusing on minimising the time and cost of the analysis, an important objective for any potential high-throughput screening system.
Although the NURD complexes purified by different laboratories vary slightly in compositions, it is generally agreed that the NURD complex contains either CHD3 (also known as Mi2-α) or CHD4 (also known as Mi2-β), HDAC1 and HDAC2, RbAp46 (also known as Rbbp7) and RbAp48 (also known as Rbbp4), MTA1/2/3, MBD3, and p66α and p66β subunits [ 14].
A third study done in four laboratories using the same platform to analyze tumor tissue blocks, cell lines, and RNA samples found that correlation within laboratories was only slightly better than correlation between laboratories, with correlations weakest for genes expressed at low levels [ 19].
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