For 2 months we followed the soil surface activity of 18 field-collected L. terrestris in an experimental laboratory population.
The team cleaned out the microbes living inside a laboratory population of punctatissima and then inserted them into the gut of the stinkbug's cousin, M. cribraria, which does not normally prey on soy plants in huge numbers.
An OARDC laboratory population was established from these initial individuals.
The laboratory population of B2 was established from field collected aphids from Wooster, OH in 2005.
Mendoza 1 was a sample of approximately 6,000 pupae taken from the Mendoza laboratory population.
In the laboratory population, both alleles are moderately abundant, and genotypic frequencies accord with expectations under Hardy-Weinberg equilibrium.
Nevertheless, an earlier study has reported precisely the same association of small body size and obligate parthenogenesis in an artificially selected laboratory population of Brachionus calyciflorus [27].
The chico1 allele is maintained as a balanced stock that has been backcrossed to the Dahomey outbred laboratory population as described in Clancy et al. (2001) [21].
DNA was extracted from 25 30 aphids of the B2 laboratory population using the OMEGA EZNA DNA tissue kit (Doraville, GA) following manufacturer's instructions.
However this is clearly not the case for our laboratory population, where no such benefit can be found, despite persistent infection.
Mendoza 2 was a sample of approximately 28,000 pupae received from the Mendoza laboratory population (G0).We assembled 73 familiesies from the Mendoza 2 colony.
