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Body weight and laboratory parameters were obtained after the day of the magnetic resonance imaging (MRI) scan.
Laboratory parameters were obtained and compared with bovine reference values [ 46].
Blood samples for the analyses of DNI and other laboratory parameters were obtained from indwelling arterial catheters or by venipuncture within the first 24 hours of ICU admission.
The following laboratory parameters were obtained: total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), triglycerides (TG), HbA1c, serum creatinine, uric acid and HAb1c.
According to the study protocol, and as described previously [ 15, 19], complete data on baseline clinical, haemodynamic, and laboratory parameters were obtained using a standardised questionnaire.
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Their model parameters were obtained from laboratory data.
Vital parameters were obtained, routine laboratory values measured, as well as acute physiology and chronic health evaluation (APACHE II) score [15], simplified acute physiology score (SAPS II) [16] and sequential organ failure assessment score (SOFA) [17] computed.
Baseline characteristics, medications and laboratory data including electrolytes and renal function parameters were obtained from all patients.
Conventionally, a laboratory-based experimental investigation requires to check all combinations of all independent parameters describing the product; hence the optimum levels of the tested parameters is obtained.
After identification of these patients, their other laboratory parameters (lipids) were obtained and their charts were reviewed for blood pressure measurements that occurred within the study time period.
All laboratory parameters were normalized at discharge.
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