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To identify novel fertile meiotic mutants on the X chromosome of Drosophila melanogaster, we undertook a large-scale screen employing a FLP-FRT mediated germline clone strategy that is analogous to the strategy used in screens FLP-FRT mediatednts on 2 L and 3 R by the Hawley Laboratory (Pagermline. 2007).
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Key information from each experiment is abstracted manually, with the only required information consisting of the ChemSpider IDs of the reactants and the product targeted in the experiment; and a link to the laboratory notebook page.
If the main text did not contain an accession ID or a link to any FLEO files, we searched existing repositories and the research group's laboratory web pages.
Biological information collected through extensive manual review of literature and unpublished information from individual laboratory web pages or databases are centralized and then undergo substantial quality checks for accuracy and precision.
This database was produced by providing a home, open-ended laboratory site and page title list to choose the titles of the open-ended laboratory.
Faculty members devote 1 h of laboratory time, multiple pages of laboratory manuals, many comments on draft papers, and significant time in face-to-face meetings to provide statistical instruction.
PCR analysis of Sry (Y-linked gene) was performed to determine maleness and Rapsn was used as an autosomal, internal control as described (Mouse Phenotypes, a Handbook of Mutatation Analysis, Cold Spring Harbor laboratory press, Chapter 3, page 40, 2005).
The authors thank Emilio Cano and Fátima Durán for laboratory assistance; R. G. Page, V. Valcárcel, A. Herrero, O. Filippi, J. J. Aldasoro, M. Escudero, M. Luceño, the MA herbarium, and particularly B. Guzmán and C. García-Verdugo for crucial plant material; J. L. Blanco, C. García-Verdugo, J. A. Calleja and M. Navascués for comments that improved the quality of the manuscript.
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