The laboratory of the Clinical Oncology, section Experimental Cancer Immunology and Therapy at the Leiden University Medical Center, is a research laboratory where the assays are performed according to SOPs, including the predefined criteria for positive responses, by well-trained personnel.
These samples were then sent to the Division of Mercury in the Laboratory of Toxicology, Section of Environment (SAMAM) of the Evandro Chagas Institute (ECI), located in the town of Ananindeua, State of Pará, Brazil.
Sex of the chicks was determined from the samples with molecular methods as described by Fridolfsson & Ellegren [34] in a molecular laboratory at the Section of Ecology, Department of Biology, University of Turku [35].
The analyses were conducted at the Laboratory of Forensic Toxicology of the Section of Legal Medicine of the University of Perugia during May 2015.
The analyses were conducted at the Laboratory of Forensic Toxicology of the Section of Legal Medicine of the University of Perugia, using GC/MSD system.
Samples were analyzed in the Analytical Chemistry Section Laboratory of the Michigan Department of Community Health.
Dried Blood Spots (DBS) were stored in individual ziplock bags containing a desiccant, and sent to the laboratory of the Department of Surgery, Oncology and Gastroenterology, Section of Oncology and Immunology, University of Padova, Unit of Viral Oncology-IOV IRCCS, Italy.
The authors thank Dr. Ge-Hung Jiang at the Section of Diagnostic Laboratory of the National Taiwan University Veterinary Hospital for identification of B. gibsoni.
For all patients, the histological diagnosis and the stage of cancer were established by assessment on paraffin sections at diagnostic laboratory of the Maddalena Hospital.
