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Based upon our analysis and our laboratory interest, some of the genes that were regulated by Mta1 with/without P53 back ground were selected for the validation using the RT-qPCR assays.
Selection of CFTR was based on our laboratory interest in regulation of that particular gene product.
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Candidate genes were selected based upon the functions and our laboratory interests.
Conversely, a laboratory interested in the formation of a given tissue, may want to place a variety of ORFs under control of a driver specific for this tissue.
Furthermore, our marker development method only relies on standard molecular biology tools and on a simple and customized bioinformatic pipeline and is thus expected to be easily and routinely useable in any laboratory interested in a fast and cheap marker development procedure.
The workflow employs noncustom, off-the-shelf equipment and is accessible to any laboratory interested in phosphopeptide analysis.
In 2008, TDR issued a public request for applications for any laboratory interested in becoming a member of a VL laboratory network to perform the VL RDT evaluation.
As no special equipment is required, this method can be applied by any laboratory interested in the analysis of DNaseI hypersensitive regions.
This technique uses commercially available, off-the-shelf components and can be easily adopted by any laboratory interested in phosphoproteomic analysis.
Alternatively, a more global approach can be taken through participation in crowdsourcing projects such as the Small World Initiative with oversight from a mentor laboratory interested in similar subjects.
Utilization of student data: Although the amount of data contributed by the students may be significant to a laboratory interested in preliminary data for a larger project, an authentic research experience should ideally include involvement in development of a peer-reviewed publication.
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