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Only for leukocytes isolated from the blood vessels of rat renal allografts undergoing fatal acute rejection, mRNA expression of CHRNA5, CHRNA9, CHRNA10, and CHRNB2 was described by our laboratory; expression of CHRM was not investigated [ 33].
Data were incorporated into the Hope Laboratory Expression Pattern Database [ 18]with details of the DNA fragment assayed, a text description of the GFP expression pattern, example images and further information about each strain generated.
Among other known A. pleuropneumoniae iron acquisition-related genes, tonB2 also showed up-regulation, while genes of the fhu operon did not show any significant change in expression, in agreement with previous work done in our laboratory; expression of fhuA is not regulated by iron [ 12].
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Recently, Dobbin et al. demonstrated that by using standardized conditions and a single technological platform (Affymetrix HG-U133A), a cancer-related (RNA from tumor tissue and cell lines), multi-laboratory expression profiling study was feasible [ 6].
To address the problem of variability between measurements originating from different laboratories, expression ratios between conditions are not calculated for merged experiments.
Although not identified as the primary biomarker in the study protocol, on the basis of preclinical results from 2 independent laboratories, expression levels of the HER3 ligand heregulin (HRG) were prospectively declared the predictive biomarker before data unblinding but after subject enrollment.
In 1981, he published a paper titled Virus and Recombinant DNA and proposed two research directions for the laboratory: gene expression in CHO cells and the virus as a vector for genetic engineering.
In previous study of our laboratory, the expression of C4H in different organs was diverse between different species.
In our laboratory, increased expression of eNOS mRNA was also proven to prevent SAH-induced vasospasm via treatment of E2 in experimental models [ 12].
It is for these reasons, cost, efficiency, flexibility and convenience, that most often laboratory protein expression prefers to utilize bacterial hosts.
In our laboratory, transient expression has been successfully used for evaluation of soybean promoter variants [ 10], but these evaluations were performed using lima bean (Phaseolus lunatus) cotyledons.
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