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Routine laboratory analysis will be made locally at the study sites.
For most ICUs the delay associated with central laboratory analysis will be unacceptable and therefore a blood gas analyzer should be the default analyzer.
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We envision that protein quantification by MS-platforms will constitute a paradigm shift in hospital laboratories worldwide; targeted analysis will be made by selected reaction monitoring "SRM" assays.
Some of these laboratories are presently running high-resolution sequencing activities in parallel with classical laboratory exercises, and understanding their cost-benefit analysis will be crucial to develop the justification required to convince stakeholders to choose a coordinated path.
HbA1c analysis will be conducted by a central laboratory (Covance, Indianapolis, Indiana, USA).
HbA1c analysis will be conducted by a central laboratory, using HPLC methodology (VII Turbo haemoglobin testing system, Bio-Rad Laboratories, HerCaliforniaifornia, USA).
Quantitative coronary analysis will be performed by an independent core laboratory.
SDS-PAGE and Western blot analysis will be completed as previously described in our laboratory [9], [10], [23].
Although more challenging, the preparation of Ag/Cu heterobimetallic complexes is underway, and their detailed analysis will be the subject of future studies from this laboratory.
Serum samples will be frozen and stored until the end of the trial when [hsTnT] analysis will be undertaken in a single batch, at a single laboratory.
The primary analysis will be a comparison of the cumulative incidence of laboratory-confirmed influenza in infants aged <6 months by treatment group (vaccinated cohort vs unvaccinated cohort).
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