Sensitivity testing at our laboratory was performed using Phoenix (Becton Dickinson) automated system.
All HOOH measurements in the laboratory were performed using the Orion-L luminometer and an acridinium ester chemiluminescence protocol (Methods S1).
Many iCa measurements in the core laboratory are performed using blood gas analyzers which are often also used in point of care testing (POCT).
The identification of S. zooepidemicus to the species level varied between the laboratories, and was performed using at least one of the following tests as shown in Table 1: APIStrep,, STR Rapid ID32, or VITEK2 GP-ID (all from bioMérieux Marcy l'Etoile, France), combined with AccuProbe Group B Streptococcus Culture ID Test (Gen-Probe, San Diego, CA, USA).
CN confirmation in three commonly used classical laboratory strains was performed using droplet digital PCR (ddPCR).
Fine milling (particle size <0.5 mm) of laboratory samples was performed using a universal cutting mill (Pulverisette 19, Fritsch, Idar-Oberstein, Germany).
The laboratory polysomnography was performed using the Compumedics Profusion Sleep 4 (Compumedics USA, Charlotte, North Carolina, USA) or the Philips Respironics Alice 5 systems.
A retrospective study of the relationship of season to the absorption of radiolead in laboratory rats was performed using data representing 305 animals from 36 experiments over 6 calendar years.
Clinical and laboratory assessment was performed using standard measures of 28 tender and swollen joint count (DAS28), rheumatoid factor, anti-cyclic citrullinated peptide antibody, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and global health visual analogue scale (VAS).
In order to assess the clinical implications of the small difference in risk scores observed between the two laboratories, ROC analysis was performed using 5-year relapse-free survival as the binary outcome metric.
Laboratory antimicrobial susceptibility testing was performed using modified Kirby- Bauer disc diffusion method which is recommended by the Clinical and Laboratory Standards Institute (CLSI) [ 7].
