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Barry Scheck and Peter Neufeld (Op-Ed, May 11) call for the creation of independent forensic testing laboratories to avoid the persistent errors that plague analyses of crime scene evidence by police laboratories.
In both laboratories to avoid potential contamination, a maximum physical separation between the pre- and post-amplification steps was used.
In areas where, in addition to TB, melioidosis and paragonimiasis are endemic, it is desirable to develop diagnostic algorithms in peripheral laboratories to avoid serious and costly mistakes.
Samples of food and samples from the index case-patient were analyzed in 2 independent laboratories to avoid any cross-contamination.
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He said it was a strict practice in the police laboratory to avoid declaring a match.
Moreover, correct interpretation of microbiological cultures depends on the capacity of the laboratory to avoid or minimize the presence of contaminants; hence, proper handling of samples and cultures (aseptic conditions, biosafety cabinet) is mandatory.
It's important that the PSA level is always determined in the same laboratory to avoid deviations due to differential measurement methods.
Alternatively, samples could be kept at body temperature (or at 37 °C in the laboratory) to avoid the loss of MSCs due to solidification of fatty components.
At the end of each year, an annual report of all data stored in the central database was sent to each participating laboratory to avoid possible discrepancies.
Each positive qPCR result was confirmed by another R. felis specific qPCR (orfB) not previously used in our laboratory (to avoid contamination with other amplicons).
Similar strict protocols were also implemented in the analytic laboratory to avoid external contamination with the target biomarkers (Ye et al. 2013).
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