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In case of blood sampling at local laboratories, the sample was sent to our clinic to avoid inter-assay differences.
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Once in the microbiology laboratory, the sample tubes were mixed up and proceeded to the planting of 100 microliters blood agar plates (Biomerieux®).
In the laboratory, the sample was aliquoted and stored at −70°C.
In the laboratory, the samples were mixed and sieved (2 mm).
In the laboratory, the samples were put into incubator with constant temperature, humidity and photoperiod similar to the field conditions.
After delivery to the laboratory, the samples were stored at the same temperature until the analysis (within 24 h).
Up on their arrival to the laboratory, the samples were stored in a refrigerator at 4 °C until they were analysed.
After being taken back to the laboratory, the samples were stored at −20 °C until being used for analysis.
Once received in the central laboratory, the samples were logged and transferred to −80°C freezer until the DNA was extracted.
Upon arrival in the laboratory, the samples were examined.
In the laboratory, the samples were dried at 105 °C until dry mass was obtained.
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