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As such, the use of transgenic lines precludes the need for other costly and labor-intensive procedures to achieve functional transgene expression in each individual experimental animal.
Real-time quantification of recombinant proteins is important in studies on fermentation engineering, cell engineering, etc. Measurement of the expression level of heterologous proteins in bacterial fermentation broth has traditionally relied on time-consuming and labor-intensive procedures, such as polyacrylamide gel electrophoresis, immunoblot analysis, and biological activity assays.
However, the application of this method is limited by the need for time-consuming and labor-intensive procedures.
It is desirable that the isolation of the RNA product and recovery of DNAzymes avoid labor-intensive procedures.
This method represents one of the least expensive, more convenient, and less labor-intensive procedures of MP isolation.
The traditional techniques for detecting and characterizing enteroviruses rely on the time-consuming and labor-intensive procedures of viral isolation in cell culture and neutralization by reference antisera [ 1, 19].
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Testing blood pulled from vessels near the heart can provide even more information, but that requires a painful, highly invasive, labor-intensive procedure.
Sanger sequencing, that has been the gold standard for mutation analysis in cancer since the 1970s, suffers from low sensitivity, complexity, and time-consuming and labor-intensive procedure.
Performance of the PCR assay could be increased to every 8 12 hours to enhance detection, but PCR is, currently, more labor-intensive procedure than is the reading of blood smears, and would require round-the-clock staffing in a challenge setting.
This is a labor-intensive procedure and requires specialized and relatively expensive microfluidics devices.
Since the segmentation, quantification, and analysis of synaptic junctions are a labor-intensive procedure, we used Espina software, a specifically developed tool that greatly facilitates and accelerates these processes (Morales et al. 2011).
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