The major reason for this is that the low abundance of aromatase in the breast requires sophisticated, time-consuming and labor intensive methodology and relatively large amounts of fresh tissue.
Despite this pioneering work, the low yields of hESC-MKs, labor-intensive methodologies, and limited in vitro functionality testing of hESC-platelets left critical questions unanswered: (1) can hESC-derived MKs and platelets be produced on a clinically relevant scale, and (2) are these platelets functional upon transfusion into a living animal?
A smaller cohort group due to the labor-intensive methodology was chosen, including females 20 25 and 35 45 y (n = 6 8) for all ethnic groups.
The MiSeq uses fluorescently tagged terminator chemistry and requires 39 hours for paired-end sequencing but has higher throughput and an associated simpler, less labor intensive library preparation methodology than the PGM.
Unfortunately, the 2-DE methodology remains labor intensive and also the subsequent gel analysis is difficult.
7, 8 However, this methodology is labor intensive and not used routinely.
Current feeding methodologies, however, are labor intensive and only semi-quantitative.
Measurement of HbA1c requires a whole-blood sample; sometimes relatively labor-intensive assay methodologies and concerns have been raised about the performance of HbA1c in certain subpopulations (25).
The latter finding is of particular important, since it supports the notion that EPCs obtained by labor intensive and time consuming cell culture methodology, thus exhibiting several functional properties of endothelial cells, are indeed related to vascular biology, and are not monocytic cells that may also be involved in inflammation.
However, the methodology is considerably more labor intensive than DNA sequencing of an mtDNA fragment, and may prove less useful in cases of highly degraded tissue samples.
