Cell labeling was monitored by fluorescence microscopy using a Nikon Eclipse E800 fluorescent microscope.
Viral labeling was monitored at 4 12 h intervals, up to 3 days (72 h) following the intramuscular injections.
The efficiency of probe labelling was monitored in a liquid scintillation analyser (1600 TR Packard).
Cy3 labeling was monitored with a Nanodrop ND-1000 spectrophotometer and was found to be between 1.2 and 1.9 pmol/μl.
The labeling was monitored qualitatively; i.e., the evaluation was based on the presence/absence of the modified peptides in 45CBS and wtCBS.
Incorporation of the fluorescein label was monitored by comparing the fluorescence with a reference strip of serially diluted nucleotide mix containing the fluorescein-11-dUTP molecules.
(xxxviii) In a similar manner, GalNAz-labeled glycoproteins in zebrafish embryos were imaged between 60 and 73 h postfertilization (hpf), and dynamic labeling was monitored in the pectoral fins, olfactory pit, and jaw.
Labeling efficiency and labeling kinetics were monitored with ITLC strips (Biodex, Shirley, NY, USA), eluted with 0.2 M citric acid (Sigma Aldrich) and evaluated with a PhosphorImager system (Perkin Elmer, Waltham, MA, USA) using OptiQuant as analysis software (Perkin Elmer).
Patients receiving open label treatment were monitored regularly and blood, urine and faecal samples were analysed using trial protocols.
Labelling efficiency and kinetics were monitored by instant thin-layer chromatography (ITLC) (Biodex, Shirley, NY, USA) eluted with 0.2 M citric acid (Sigma Aldrich).
