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Fatty acid spin labels were incorporated into vesicles, composed of the single chain non-ionic surfactant octaoxyethylenelaurate-ester (PEG-8-L), the sucrose laurate-ester l-595 and cholesterol sulfate (CS) to monitor local dynamic properties of lipid molecules in vesicle bilayers and to study the elasticity of vesicle bilayers.
Fluorescent labels were incorporated directly during cDNA synthesis using the ChipShot direct labeling and cleanup system according to the manufacturer's instructions (Promega, Madison, WI).
Cy3 and Cy5 (Amersham) labels were incorporated into reference and experimental cDNA (green and red, respectively) via first strand cDNA synthesis.
In this report, we describe the REDOR NMR analysis of FemA cell walls into which four specific labels were incorporated: d-[1-C]alanine, l-[3-C]alanine, [2-C]glycine, and l-[5-F]lysine.
Labels were incorporated at the stage of cDNA synthesis.
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Both labels are incorporated into cells that are actively synthesizing DNA.
Similarly, 24 nmols of the Cyanine 5-CTP (Cy5; PerkinElmer) fluorescent label were incorporated to 400 ng sample of Human Universal Reference RNA (Stratagene, USA); in addition, dye-swap replicate amplification were performed.
However, this method only provides a rough estimate of fractional hepatic fatty acid synthesis since 3H2O-derived label is incorporated into multiple positions in fatty acids by different metabolic pathways.
Normal lysine and arginine were incorporated as light labels while for heavy labeling the isotopes were 8 and 10 Da heavier.
Normal lysine and arginine were incorporated as light labels; 6 Da (Dalton) heavier isotopes of the same amino acids were used as medium labels; while for heavy labeling the isotopes were 8 and 10 Da heavier.
In order to assess consumers' intention to buy apparel items with a sustainability label, five sustainability labels that are currently available on apparel products in the U.S. market were incorporated to PI items (Targosz-Wrona 2009).
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