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Extract Bandicoot's indicators for the random set of users whose CDRs and labels were collected.
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Demographics, tumor stage and size (T), nodal status (N), estrogen receptor (ER), grading (G) progesterone receptor (PR) and HER2/neu status, and tumor proliferation measured by ki67 labeling were collected.
Since the cells used for reciprocal labeling were collected independently, we pooled the data from reciprocal mixes by expressing the change in frequency in terms of the same clone.
At the end of each experiment, a wide-field (500 µm × 500 µm) image stack of Fluo-4 cellular labeling was collected from the brain surface down to ∼350 µm deep.
The labeled viruses were collected and subjected to viral titration assays using focus formation assays or RNA measurements by qRT-PCR.
Heavy labeled cells were collected and lysed.
After labeling, samples were collected in every 24 hours for 120 hours and the fluorescence intensity was measured.
After metabolic labeling, cells were collected in 0.1 ml of sample buffer [160 mM Tris HCl pH 6.8; 2% SDS; 11% glycerol; 0.1 M DTT, 0.033% bromophenol blue].
Images from the stained nuclei and antibody labeled bacteria were collected at five random fields within each well, and further analyzed using image J NIH software; l images within each channel were set with the same parameters.
Subsequently, the cell suspension was added, rinsed and the columns removed from the quadroMACS; labeled cells were collected in a new tube by rinsing with MACS buffer and the supplied plunger.
For multicolour labelling, images were collected sequentially.
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