From the centre of mass of the signal produced by the label, we determined the position of the head as a function of time revealing discrete 74 nm steps at 1000 frames/s imaging speed with ∼4 nm positional precision.
Although the hairpin included two dG sites, using 2D NMR in conjunction with site-specific labeling we determined only G15 in the GAA hairpin promoting motif formed a covalent adduct with Dox.
To quantify the dynamic behavior of labeled channels we determined instantaneous diffusion coefficients (Di_inst) along trajectories.
In summary, with the use a mouse model for tracking cell fates and a mouse label-retaining assay, we determined that, in the prostate, luminal cells are derived from a basal lineage and that slowly cycling cells, which may represent adult prostate stem cells, reside in the basal cell compartment.
Using mutagenesis and site-directed labeling with fluorescence probes, we determined that MVT alters actin interprotomer contacts and dynamics, which presumably reflect the observed changes in bending persistence length.
Using double label in situ hybridization, we determined that KIT is expressed in a subset of TRPM5 cells (encompassing sweet, bitter, and umami taste cells) (Figure 4B D).
Using a fluorescently labeled anti-PSMA aptamer analogue, we determined that there are approximately 213 anti-PSMA aptamer analogues per gold nanoparticle.
In the same lesions that we determined the bromodeoxyuridine labeling indices, we also counted apoptotic cells based on their morphology.
With this labeling we can determine the number of true positives (TPs), FPs, false negatives (FNs) and true negatives (TNs).
Once all lines in a block are labeled by our CRF model, we determine the attribute of the block by majority voting.
