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To achieve this, the percentage of raters' labels (excluding abstentions) agreeing with the original author-based labels was calculated, both overall and for each item separately.
Relative quantification of proteins using the TMT labels was calculated using the tandem mass spectrometry scans as the ratio of the areas under the peaks at 126, 127, 128, 129, 130 and 131Da, which correspond to the representative masses of the TMT reagents.
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In MRMR, the mean value of all mutual information values across individual features and class labels are calculated as the max-relevance values.
For each of the 10 datasets D1, D2,…, D10, Cramer's coefficients of the features and samples' class labels were calculated.
Protein concentration and degree of labelling was calculated by measuring absorbances with a NanoDrop 2000/2000c Spectrophotometer (Thermo Fisher Scientific) using extinction coefficients provided by the dye manufacturer.
Degree of labeling was calculated to 3 mole dye per mole protein.
The degree of labeling was calculated and found to be 3.11 mole of Alexa Fluor® dye per mole of transferrin.
The density of pMAPK labeling was calculated as the ratio between the total number of pMAPK labeled neurons and the contour area (mm2) of each LA subnucleus.
After removal of free dye, and buffer exchange to 50 mM phosphate buffer pH 7.0, the efficiency of protein labeling was calculated as mole of dye per mole of protein according to manufacturer's protocols (Invitrogen).
Fraction labeled was calculated as (50% N intensity)/(N intensity + 50% N intensity).
The percentage of erroneous responses for each emotional label was calculated as the rate of erroneously selected labels in all trials for that facial expression.
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