McCartney, M. D., Wood, T. O. & McLaughlin, B. J. Freeze-fracture label of functional and dysfunctional human corneal endothelium.
Additionally, domains sharing the same ocular dominance as the neurons at the injection sites were visualized by 2-deoxyglucose autoradiography to allow comprehensive labeling of functional domains in regions far from the injection sites.
Besides the selective labelling of functional terminals by their antibody uptake it enables visualization of synaptic exo- and endocytosis cycles according to the stimulation dependent change in fluorescence.
For live-cell antibody labelling of functional terminals we used a monoclonal antibody directed against the intra-vesicular domain of synaptotagmin-1.
Moreover, the labelling of functional foods is far from informative, providing scanty details about nutritional value, storage, and cooking recipes.
For mitotracker Red labeling of functional mitochondria, cells were incubated with mitotracker Red CMXRos (200 nM, 10 min, Molecular Probes, Invitrogen) before PFA fixation.
For the labelling of functional blood vessels in tumors, mice were anesthetized using 75 mg/kg ketamine and 20 mg/kg xylazine, followed by the injection of 100 μg of biotinylated- Lycopersicum esculentum lectin (Vector Laboratories, Burlingame, CA) via the tail vein of the mice.
The science behind the promising labels of such functional foods, or "foods [that] are not only intended to satisfy one's hunger but also to eliminate nutrition related diseases" according to Grand View Research, often falls short, however.
The anatomical localization and labeling of the functional data was determined by both Talairach coordinates and three radiologists.
Literature labelling of our functional molecular networks confirmed multiple previous (molecular) observations in the AD literature and revealed many new ones.
