This labeling is specific, and it shows that the Mr 64,000 protein contains the ligand binding site of the alpha 2-adrenergic receptor.
To-gether this showed that labeling is specific for the presence of PilQ in the indentations.
This labeling of rod terminals is unlikely to be a fixation artifact, since the sections were lightly fixed and labeling was specific to synaptic terminals.
Only a subpopulation of taste cells exhibited antibody labeling and negative controls were blank, indicating that the antibody labeling was specific.
To test whether the labeling was specific for the presence of the indentations, membranes from the outer membrane enriched fractions of the wild type N.gonorrhoea MS11A strain and the pilQ deletion mutant strain were mixed and immobilized on a grid.
Yet both Western blot data suggest labeling is specific to the MR. We next incubated the antibody MA1-620 withethe peptide used to generate the second MR antibody (rMR1-18 1D5) (Fig. 3C).
This labeling was abolished when the blocking peptide was incubated with the primary antibody (see Figure 3E) and immunoreactivity was not readily apparent below the taste bud (see Figure 3B), so we concluded the antibody labeling is specific and that GABA Aα1 expression is not restricted to the taste bud.
Control sections revealed little immunoreactivity in the nucleus or cytosolic organelles, suggesting that the primary antibody labeling was specific to the GR or MR. In sum, glutamatergic and GABAergic neurons both contained MR-ir and GR-ir in nuclear and non-nuclear structures.
Primary and secondary antibody controls indicated that the primary antibody was specific to the antigen and the label was specific to the primary antibody (data not shown).
Punctate labeling is diminished in images of tissue near the tracks of probes perfused with aCSF, which also exhibit diffuse TH labeling: control experiments did not reveal nonspecific binding, which indicates that the diffuse labeling is specific binding.
