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Therefore, an automated and objective computation of the correlation between labels and microarray data is needed to identify correlated clinical parameters.
Pioneering work by Wang and Bucan [ 20] has shown that the use of biological labels and microarray data analysis tools can facilitate the interpretation and priorization of candidate genes in WGAS.
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About 2 μg RNA was isolated from anthers and used for RNA labeling and microarray hybridization.
DNA labelling and microarray hybridization was conducted by staff at the W.M. Keck Facility at Yale.
cDNA labelling and microarray hybridization and was done exactly as previously described [7], using oligo-based microarrays (TIGR).
Microarray design and preparation, sample labeling, and microarray hybridization and scanning services were performed by the Harvard Partners Center for Genetics and Genomics (now Partners Center for Personalized Genetic Medicine).
RNA sample amplification, dye labeling, and microarray hybridization was conducted as part of Community Collaboration under the Songbird Neurogenomics Initiative and used a universal reference design [23].
Only the best single cell cDNAs (10 µg of each), as determined by RT-PCR and Southern blot, were selected for labeling and microarray hybridization.
We are thankful to Beth Johannessen regarding cell culture work and RNA purification and to Hua My Hoang regarding RNA purification, nucleic acid labeling and microarray assays.
To avoid systematic bias due to batch/lot and processing variability, bariatric surgery samples and control samples were balanced and randomized in each of the following steps: adipose tissue homogenization, RNA extraction, labeling, and microarray hybridization.
We thank Marja Nieuwland for mRNA isolation, labeling and microarray hybridization; members of our laboratory, Paul Koppen, Maarten Fornerod and Bernike Kalverda for helpful discussions; and Pam Geyer for providing SU HW) antibody.
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