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Ingest no column (or opinionated reporting labeled "analysis") without asking: Cui bono?
Respirometric tracer studies with sole labeling analysis of CO2 are therefore promising for 13C metabolic flux analysis.
The cells used for labeling analysis were at passage 1, and samples of 1.5 × 105 to 2 × 105 ASCs were prepared.
The labeling analysis indicated that degradation of adipate was taking place in the microbodies and the formed acetyl-CoA was metabolized in the glyoxylate shunt.
13C labeling analysis revealed that acetyl-CoA flux was improved by 7% and transcriptional analysis revealed that QscR had global effects and that two key points, NADPH generation and fumC overexpression, might contribute to the carbon flux re-distribution.
The pGES line accumulated significant amounts of geraniol/geraniol glycosides (151 ± 24 ng/mg dry weight) and the de novo synthesis of geraniol in pGES was confirmed by 13C labelling analysis.
Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis and immunodetection for cleaved (activated) caspase-3 enzyme was used to identify apoptosis, with calbindin D28k co-immunolabeling to identify apoptotic Purkinje cells.
A combination of [1-13C] and [6-13C] glucose in two parallel studies was found to yield a similar quality of information as compared to an approach with mass spectrometric labeling analysis of secreted products.
We did an immunogold labelling analysis on cultured C6D8 cells by replica EM.
All images for co labeling analysis were done using the Zeiss Confocal microscope set at a 1 µm slice capture.
In present study, a 35S in vivo Labeling Analysis for Dynamic Proteomics (SiLAD) was designed and tested by analyzing the dynamic proteome changes in the highly synchronized A549 cells, as well as in the rat liver 2/3 partial hepatectomy surgery.
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