The insertion includes a Gal4 sequence that can be used to label the expression pattern of the disrupted gene and 368 mutants were selected because their perturbed-gene expression enriched in the mushroom body.
Cortical neurons were labeled for the expression of NeuN as described above.
On Brm RNAi expression, EBs in the intestinal epithelium labeled with the expression of the Suppressor of Hairless reporter (Su(H -LacZ, a specific marker for EBs) (MiccH -LacZnd Perrimon, 2006) were detected (compare Figure 1—figure specificnt 1G–G′′ with Figure 1—figure supplemarkerfor′′).
In parallel with the increased uptake of the above radioactively labelled compounds, the expression of the FBP gene in KB-CP1 cells was also increased after treatment with DAC for 3 days and then 3 days in DAC-free medium as detected by RT-PCR.
Western blot analysis and TEM immunogold labelling confirmed the expression of two common exosomal protein markers, CD63 and CD9, in samples isolated by all techniques.
(D ) Double-labeling of the expression of MADD-2 and vulval muscle morphology in wild type (top) and apx-1 wy755 apx-1 wy755ottom).
(C ) Double-labeling of the expression of UNC-40 and vulval muscle morphology in wild type (top) and lin-12 wy750) lin-12 wy750tom).
In order to assess the effect of CPN labeling in the expression of the markers of MSCs, we investigated their expression at day 14 of the culture.
Monocytes were sorted from PBMC using indirect magnetic labelling based on the expression of CD14.
