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Cells were mock-treated, or treated with the drug and successful disruption of actin filaments confirmed by confocal microscopy after labelling the cells with Alexa-488 labelled phalloidin (data not shown).
The time-course of ceramide formation in the RBC membranes was estimated by labelling the cells with an anti-ceramide antibody and sorting the cells by FACS (Fig. 6B).
When I went to see Rakic at Yale, he spent a long time disputing some of her latest findings; he could find no evidence of neurons in the cortex, and he is convinced that Gould (together with her Princeton colleague Charles Gross) made a mistake in labelling the cells.
After labelling the cells were embedded in Mowiol containing Dabco antifade reagent (Sigma).
Morphological determination of levels of apoptosis was performed by labelling the cells with the nuclear stain Hoechst 33258 and visualisation by fluorescence microscopy.
After labelling, the cells were washed and analysed by flow cytometry (FacsCalibur, BD Pharmingen).
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Ethidium Bromide (Invitrogen) at a dilution of 1∶50,000 was used for labelling the cell nuclei.
But Dr. Frisen realized several years ago that nuclear weapons tested in the atmosphere until 1963 had in fact labeled the cells of the entire world's population.
The problem was how to label the cells with fluorescent dyes to continuously monitor their growth and division.
The chip is made from a transparent plastic, which means that researchers can label the cells with different markers and use automated programs to analyze them.
We then labeled the cells with SBA-488 before fixing and immunostaining, without permeabilization, for RSV F and G (Fig. 1c).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com