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A 30/1 labelling ratio corresponding to 100% labelling yield was assumed for further experiments.
For the anti H3 antibody the labelling density was of 86 particles per µm2 (p/µm2) in HC and of 42 p/µm2 in EC yielding a HC/EC labelling ratio of 2. This ratio is significantly lower than the HC/EC nucleosome density ratio of at least 5 found by tomography suggesting that the accessibility of this epitope is affected by the nucleosomal packing.
In addition, mass spectrometric analysis was used to assess the labelling ratio.
The labelling ratio was estimated spectroscopically by measuring the absorbance at 280 nm for the SIP (assuming that a 1 mg ml−1 of SIP solution gives absorption of 1.4 U at 280 nm) and at 422 nm for the PS (ɛ=159 100 M−1 cm−1).
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The results show that our algorithm outperforms the corresponding unsupervised and semi-supervised clustering methods even if the wrongly labeled ratio reaches 30%.
Only an increased aspartate/PEP-labelling ratio was observed in LC acclimated WT cells (Table S1B), which indicates PEP carboxylase activation under LC conditions.
We also examined the effects of the Aβ/QD labeling ratio, in conjunction with SDS, on QDAβ oligomerization.
The labeling ratio of Cy5 to antibody (Cy5∶antibody) was calculated as described [29] and was close to 1∶1.
β-Gal was labeled with biotin using EZ-Link Sulfo-NHS-Biotinylation Kit purchased from Pierce (labeling ratio: ∼two biotin per enzyme molecule).
The difference in fluorescence intensities is a result of different fluorescent labeling of TcdA and TcdA1 1874 (TcdA-PE/Cy5 and TcdA1 1874-Atto488) as well as of different labeling ratio due to size of toxins.
A given labeling ratio of cell proliferation may arise from two distinct scenarios: either all cells proliferate at a certain rate or subsets of cells proliferate at a higher rate.
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